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Based on this image's title: “Reactions catalyzed by urease and urea amidolyase. The two reactions ...”
+In+the+reaction+catalyzed+by+argininosuccinate+synthetase%2C+the+second+nitrogen+enters+from+aspartate.+Activation+of+the+ureido+oxygen+of+citrulline+in+step+1+sets+up+the+addition+of+aspartate+in+step+2..jpg)
+Urea+cycle+and+reactions+that+feed+amino+groups+into+the+cycle.+The+enzymes+catalyzing+these+reactions+(named+in+the+text)+are+distributed+between+the+mitochondrial+matrix+and+the+cytosol.+One+amino+group+enters+the+urea+cycle+as+carbamoyl+phosphate%2C+formed+in+the+matrix%3B+the+other+enters+as+aspartate%2C+formed+in+the+matrix+by+transamination+of+oxaloacetate+and+glutamate%2C+catalyzed+by+aspartate+aminotransferase.+The+urea+cycle+consists+of+four+steps.+1+Formation+of+citrulline+from+ornithine+and+carbamoyl+phosphate+(entry+of+the+first+amino+group)%3B+the+citrulline+passes+into+the+cytosol.+2+Formation+of+argininosuccinate+through+a+citrullyl-AMP+intermediate+(entry+of+the+second+amino+group).+3+Formation+of+arginine+from+argininosuccinate%3B+this+reaction+releases+fumarate%2C+which+enters+the+citric+acid+cycle.+4+Formation+of+urea%3B+this+reaction+also+regenerates+ornithine.+The+pathways+by+which+NH4%2B+arrives+in+the+mitochondrial+matrix+of+hepatocytes+were+discussed+in+Section+18.1..jpg){kind=spacer}
